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Skin-tissue IL-22 concentrations across the experimental groups.

Journal: Frontiers in Medicine

Article Title: Effects of topical Averrhoa bilimbi leaf extract cream on IL-22 and filaggrin in a murine model of atopic dermatitis

doi: 10.3389/fmed.2026.1883375

Figure Lengend Snippet: Skin-tissue IL-22 concentrations across the experimental groups.

Article Snippet: Cutaneous IL-22 concentrations were quantified using a mouse IL-22 enzyme-linked immunosorbent assay kit (Elabscience, catalog no. E-EL-M2446, Wuhan, China) according to the manufacturer's instructions.

Techniques: Control, Positive Control

Skin-tissue IL-22 concentrations after topical administration of A. bilimbi leaf extract cream in a mouse model of atopic dermatitis.

Journal: Frontiers in Medicine

Article Title: Effects of topical Averrhoa bilimbi leaf extract cream on IL-22 and filaggrin in a murine model of atopic dermatitis

doi: 10.3389/fmed.2026.1883375

Figure Lengend Snippet: Skin-tissue IL-22 concentrations after topical administration of A. bilimbi leaf extract cream in a mouse model of atopic dermatitis.

Article Snippet: Cutaneous IL-22 concentrations were quantified using a mouse IL-22 enzyme-linked immunosorbent assay kit (Elabscience, catalog no. E-EL-M2446, Wuhan, China) according to the manufacturer's instructions.

Techniques: Tissue, Cream

Post-hoc Dunn test with Benjamini–Hochberg FDR correction for IL-22 levels.

Journal: Frontiers in Medicine

Article Title: Effects of topical Averrhoa bilimbi leaf extract cream on IL-22 and filaggrin in a murine model of atopic dermatitis

doi: 10.3389/fmed.2026.1883375

Figure Lengend Snippet: Post-hoc Dunn test with Benjamini–Hochberg FDR correction for IL-22 levels.

Article Snippet: Cutaneous IL-22 concentrations were quantified using a mouse IL-22 enzyme-linked immunosorbent assay kit (Elabscience, catalog no. E-EL-M2446, Wuhan, China) according to the manufacturer's instructions.

Techniques: Comparison, Positive Control, Control

Pim1 promotes Th17 cell differentiation in vitro. (A) Relative mRNA levels of Pim1 during Th17 cell differentiation ( n = 9). (B) Relative protein levels of pSTAT3, RORγt, and Pim1 during Th17 cell differentiation ( n = 9). (C and D) Frequency of Th17 cells among CD4 + cells after AZD1208 treatment ( n = 9). (E) Relative protein levels of Pim1 among CD4 + T cells overexpressing (OE) vector or Pim1 ( n = 9). (F) Frequency of Th17 cells in CD4 + cells after overexpressing vector or Pim1 ( n = 9). (G) Relative protein levels of RORγt and pSTAT3 after AZD1208 treatment or Pim1 overexpression ( n = 9). (H and I) Relative mRNA levels of Th17-cell-associated pathogenic genes after AZD1208 treatment (H) or Pim1 overexpression (I) ( n = 9). (J and K) Concentration of IL-17A, IL-17F, IL-22, and GM-CSF in the cell supernatant after AZD1208 treatment (J) or Pim1 overexpression (K). (L) Frequencies of Th1, Th2, and Treg cells among CD4 + T cells after treatment with AZD1208 ( n = 9). The data were statistically analyzed via one-way ANOVA, followed by Bonferroni’s post hoc comparisons (A to D) and paired t test (E to L).

Journal: Research

Article Title: Pim1 Serves as a Therapeutic Target for Inflammatory Arthritis via Mitochondrial Metabolism and Th17 Cell Differentiation

doi: 10.34133/research.1137

Figure Lengend Snippet: Pim1 promotes Th17 cell differentiation in vitro. (A) Relative mRNA levels of Pim1 during Th17 cell differentiation ( n = 9). (B) Relative protein levels of pSTAT3, RORγt, and Pim1 during Th17 cell differentiation ( n = 9). (C and D) Frequency of Th17 cells among CD4 + cells after AZD1208 treatment ( n = 9). (E) Relative protein levels of Pim1 among CD4 + T cells overexpressing (OE) vector or Pim1 ( n = 9). (F) Frequency of Th17 cells in CD4 + cells after overexpressing vector or Pim1 ( n = 9). (G) Relative protein levels of RORγt and pSTAT3 after AZD1208 treatment or Pim1 overexpression ( n = 9). (H and I) Relative mRNA levels of Th17-cell-associated pathogenic genes after AZD1208 treatment (H) or Pim1 overexpression (I) ( n = 9). (J and K) Concentration of IL-17A, IL-17F, IL-22, and GM-CSF in the cell supernatant after AZD1208 treatment (J) or Pim1 overexpression (K). (L) Frequencies of Th1, Th2, and Treg cells among CD4 + T cells after treatment with AZD1208 ( n = 9). The data were statistically analyzed via one-way ANOVA, followed by Bonferroni’s post hoc comparisons (A to D) and paired t test (E to L).

Article Snippet: Human IL-17A enzyme-linked immunosorbent assay (ELISA) kit (Elabscience, #E-EL-H5812), human IL-17F ELISA kit (Elabscience, #E-EL-H4193), human IL-22 ELISA kit (Elabscience, #E-EL-H0106), and human GM-CSF ELISA kit (Elabscience, #E-EL-H0081) were used.

Techniques: Cell Differentiation, In Vitro, Plasmid Preparation, Over Expression, Concentration Assay